Luciferase assay protocol promega Linear results are seen over at least eight orders of magnitude of enzyme 6 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM046 · Revised 9/23 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM058 · Revised 1/23 The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, produced by the luciferase reaction, which has a half-life of greater than five hours. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM369 · Revised 1/22 The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture 3. For detailed instructions Luciferase Assay Systems INSTRUCTIONS FOR USE OF PRODUCTS E1483, E1500, E1501, E1531, E4030, E4530 AND E4550. HiBiT is an 11-amino-acid peptide tag that 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM426 · Revised 2/24 A protocol for high-throughput quantitation of luciferase expression in mammalian cells, by processing of 96- and 384-well plates. Literature # TM329. The Luciferase Assay System incorporates coenzyme A (CoA) for improved kinetics, allowing greater enzymatic turnover and resulting in increased light intensity that is nearly constant for High- and ultrahigh-throughput quantitation of luciferase expression in mammalian cells requires sensitive reagents. Literature # TM356. For Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 Figure 2. In addition, researchers must either: (1) use luminescent 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM453 · Revised 3/24 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM369 · Revised 1/22 %PDF-1. Ultra-sensitive detection of firefly and NanoLuc® luciferase activities in a single sample. N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551. Renilla and firefly luciferase luminescence were The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. N1610, N1620, N1630, N1650, N1521, 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM426 · Revised 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 A protocol for high-throughput quantitation of luciferase expression in mammalian cells, by processing of 96- and 384-well plates. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM058 · Revised 1/23 The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System allows sensitive detection of firefly and NanoLuc luciferases. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 using the Dual-Glo® Luciferase Assay System (Cat. The Renilla Luciferase Assay System is designed to provide a fast and sensitive method of detecting sea pansy 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM369 · Revised 1/22 The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. The Kinase-Glo® Assay reactions. This assay provides signal half-lives of over 5 hours in 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. The ONE-Glo™ EX Assay provides high 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www. A new luciferase substrate has enabled Promega to create a novel E1941). Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) PPI dynamics can be followed in real time inside living cells using the Nano-Glo® Live Cell Assay System, a nonlytic detection reagent that contains the cell-permeable substrate furimazine. With the Dual The Nano-Glo® Live Cell Reagent is a nonlytic detection reagent that can be used to monitor luminescence from NanoLuc® luciferase or NanoBiT® assays in living cells. Instructions for Use of Product(s) E2920, E2940, E2980. The reagent is prepared by mixing 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM369 · Revised 1/22 The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. com 2. Preparation of the Reconstituted Reagent Add one volume of 100X Renilla-Glo™ Luciferase Assay Substrate to 100 volumes of Renilla-Glo™ 2 Promega Corpora on · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM432 · 3/15 www. # E1980). Promega has several series 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM701 · Revised 10/24 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 The ONE-Glo™ Luciferase Assay System employs new assay chemistry to provide a robust, homogeneous assay. The add-read-add-read, or “Stop & Glo”, format is amenable Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www. 4 %忏嫌 1613 0 obj > endobj xref 1613 24 0000000016 00000 n 0000002732 00000 n 0000002946 00000 n 0000002983 00000 n 0000004344 00000 n 0000004889 00000 n Luciferase Assay Systems INSTRUCTIONS FOR USE OF PRODUCTS E1483, E1500, E1501, E1531, E4030, E4530 AND E4550. 4 %âãÏÓ 243 0 obj /Linearized 1 /O 248 /H [ 1794 637 ] /L 234184 /E 70555 /N 17 /T 229205 >> endobj xref 243 45 0000000016 00000 n 0000001269 00000 n 0000001426 00000 Less than 10–20 moles of luciferase have been detected under optimal conditions (2). In the DLR™ Assay, the activities of firefly ( Photinus pyralis ) and 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM058 · Revised 1/23 Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www. com Part# In the NanoDLR™ Assay protocol, firefly luciferase activity is measured first using the ONE-Glo™ EX Luciferase Assay Reagent. ONE-Glo™ EX Luciferase Nano-Glo® Dual-Luciferase Reporter Assay System. Literature # TM055. A. The components of the DLR™ 1000 Assay System are identical in formulation to those provided with the DLR™ Assay System (Cat. com Standard Protocol for the Steady-Glo® Assay System Steady-Glo® Bio-Glo-NB™ TCK Luciferase Assay System PROMEGA CORPORATION • 2800 WOODS HOLLOW ROAD • MADISON, WI 53711-5399 USA • TELEPHONE 608-274-4330 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM292 · Revised 8/23 consiste DG ® Luciferase A S. C R Rsponse R . The add-read-add-read, or “Stop & Glo”, format is amenable 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 The Nano-Glo® HiBiT Lytic Detection System sensitively quantifies HiBiT-tagged proteins in cell lysates using a simple add-mix-read assay protocol. The reagent is prepared by mixing Figure 2. # G7941): 1. Renilla Luciferase Assay System has comparable sensitivity to the Luciferase Assay System (firefly luciferase) and 100-fold greater sensitivity than a published method for assaying 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM292 · Revised 8/23 Nano-Glo Dual-Luciferase Reporter Assay (NanoDLR) System Quick Protocol, FB189 Author: Promega Corporation Subject: Nano-Glo Dual-Luciferase Reporter Assay \(NanoDLR\) The Bio-Glo™ Luciferase Assay System provides a highly sensitive Bio-Glo™ Luciferase Assay System Quick Protocol PDF (361 KB) – English. For kits E1500, E1501, E4030, 3. Preparation of the Reconstituted Reagent Add one volume of 100X Renilla-Glo™ Luciferase Assay Substrate to 100 volumes of Renilla-Glo™ A protocol for ultrahigh-throughput quantitation of luciferase in mammalian cells. The assay provides a homogeneous chemistry with high sensitivity and approximately 30-minute signal 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM369 · Revised 1/22 Instructions for Use of Product(s) E2710, E2720, E2750. com Part# 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 The assay consists of a genetically engineered Jurkat T cell line that expresses a luciferase reporter driven by an NFAT-response element (NFAT-RE). With the Dual Less than 10-20 moles of luciferase have been detected under optimal conditions. The TCR/CD3 Effector Cells (IL-2) are provided in a thaw High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. PROTOCOL Quick 1. # E2920) and a MicroLumatPlus LB96V luminometer (Berthold). Numerous configurations of pGL4 Vectors are available, 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM426 · Revised 2/24 The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. com TM463 · Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Promega has several series Less than 10-20 moles of luciferase have been detected under optimal conditions. For additional protocol information consult Technical Less than 10-20 moles of luciferase have been detected under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 A protocol for a luminescent cytochrome P450 activity assay. Light is produced by converting the chemical energy of In the NanoDLRTM Assay protocol, firefly luciferase activity is read first (after addition of the ONE-GloTM EX Luciferase Assay Reagent). # E1910, E1960; 105ml for Cat. com Part# High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Promega has several series of firefly and Renilla luciferase vectors, pGL4, pGL3 and pRL,designed for use with the DLR™ Assay Systems. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM292 · Revised 8/23 A protocol for ultrahigh-throughput quantitation of luciferase in mammalian cells. Figure 2. The Dual-Glo® Luciferase Assay System is designed to allow high-throughput analysis of mammalian cells Nano-Glo® Luciferase Assay System. This assay provides signal half-lives of over 5 hours in This Quick Protocol provides instructions for the Bio-Glo™ Luciferase Assay System designed for use with the quantitative MOA-based Reporter Bioassays using firefly luciferase reporter. ATP remaining at the time that the reagent is added is used 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM292 · Revised 8/23 A protocol for the detection and quantification of total The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total Luciferin-NT, to Luciferase Assay Systems INSTRUCTIONS FOR USE OF PRODUCTS E1483, E1500, E1501, E1531, E4030, E4530 AND E4550. In the DLR™ Assay, the activities of firefly ( Photinus pyralis ) and Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 3 www. Literature # TM058. The Steady-Glo® Luciferase Assay Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase. For detailed instructions Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www. We have a product guide to help you choose the right Luciferase Reporter Assay. Add-mix-measure assay for NanoLuc® Luciferase with >2 hour half-life in most applications. The TCR/CD3 Effector Cells Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. The vectors may be used to co This Quick Protocol provides instructions for the Bio-Glo-NB™ TCK Luciferase Assay System designed for use with HiBiT Target Cell Killing (TCK) Bioassays. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla A protocol for a vector containing five copies of an NF-kappaB response element (NF-kappaB-RE) that drives transcription of the luciferase reporter gene luc2P, a synthetically derived luciferase Instructions for Use of Product(s) E2920, E2940, E2980. The Renilla-Glo™ Luciferase Assay System is a single-addition reagent that generates a glow-type signal with The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System allows sensitive detection of firefly and NanoLuc luciferases. Renilla-Glo™ Luciferase Assay Protocol 3. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase. In addition, researchers must either: (1) use luminescent The assay consists of a genetically engineered Jurkat T cell line that expresses a luciferase reporter driven by an IL-2 promoter. primary cells, complex assay protocols and assay reagents not qualified for these protocols. Firefly luciferase is a 61kDa Instructions for Use of Product(s) E2810, E2820. promega. The add-read-add-read, or “Stop & Glo”, format is amenable 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM046 · Revised 9/23 The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. com Part# The ONE-Glo™ Luciferase Assay System employs new assay chemistry to provide a robust, homogeneous assay. Store at –20°C (≤1 month) or The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. . Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression The Luciferase Assay System is substantially improved over conventional assay methods in both sensitivity and simplicity (2,6–8). The derivatives are not substrates for luciferase but are converted by cytochrome P450s to luciferin, Please see P450-Glo™ 2 Promega Corpora on · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM432 · 3/15 www. The assay provides a homogeneous chemistry with high sensitivity and approximately 30-minute signal 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM292 · Revised 8/23 The ONE-Glo™ EX Assay offers many of the same benefits of the ONE-Glo™ Assay, including use of 5´-fluoroluciferin substrate and an add-mix-read, or homogeneous, protocol. However, 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB281 · Revised 5/24 E1941). Thaw the Bio-Glo™ Luciferase Assay Buffer in a refrigerator overnight or in a room temperature water Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. C Dual-Glo ® Luciferase Assay System Technical Manual #TM0 . This extended half-life eliminates the 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM426 · Revised 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 2 Promega Corpora on · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM432 · 3/15 www. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of Less than 10-20 moles of luciferase have been detected under optimal conditions. Specifications. The Dual-Glo® Luciferase Assay System is designed to allow high-throughput analysis of mammalian cells Luciferase Assay System (firefly luciferase) and 100-fold greater sensitivity than a published method for assaying Renilla luciferase. The reagent is prepared by mixing This Quick Protocol provides instructions for the Bio-Glo-NBTM TCK Luciferase Assay System designed for use with HiBiT Target Cell Killing (TCK) Bioassays. The ONE-Glo™ EX Assay provides high LAR II: Resuspend the lyophilized Luciferase Assay Substrate in Luciferase Assay Buffer II (10ml for Cat. Preparation of the Reconstituted Reagent Add one volume of 100X Renilla-Glo™ Luciferase Assay Substrate to 100 volumes of Renilla-Glo™ Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. Steady-Glo® Bright-Glo™ Luciferase Assay System 100ml E2620 Each system contains sufficient reagents to perform 1,000 assays of 100µl each. com TM490 · 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM058 · Revised 1/23 The ONE-Glo™ EX Luciferase Assay System quantitates firefly luciferase expression in multiwell plates using a simple add-mix-read protocol. Se S . ONE-Glo™ EX Luciferase The ONE-Glo™ Luciferase Assay System employs new assay chemistry to provide a robust, homogeneous assay. com † Phone 608-274-4330 or 800-356-9526 † Fax 608-277-2601 RenillaLuciferase Assay Protocol RenillaLuciferase Assay System INSTRUCTIONS FOR USE 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised Nano-Glo® Dual-Luciferase Reporter Assay System. Linear results are seen over at least eight orders of magnitude of enzyme Instructions for Use of Product(s) E7110, E7120. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM292 · Revised 8/23 The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. The ONE-Glo™ Luciferase Assay System(a,b)employs new assay The Dual-Glo® Luciferase Assay System(a,b) is designed to allow high-throughput analysis of mammalian cells containing genes for firefly and Renilla luciferases grown in 96- or 384-well High-throughput quantitation of luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. You are viewing: G7940 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 3 www. The ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay combines luminescent and fluorescent assay 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 3. A new luciferase substrate has enabled Promega to create a novel See additional protocol information in Technical Manual #TM051, available online at: www. The kinase reaction is conducted under the appropriate conditions. # E1910 and E1960). N1110, N1120, N1130, N1150. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 Assay protocols. Nano-Glo® Dual-Luciferase Reporter Assay System. A new luciferase substrate has enabled Promega to create a novel In the NanoDLR™ Assay protocol, firefly luciferase activity is measured first using the ONE-Glo™ EX Luciferase Assay Reagent. The detection The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. For kits E1500, E1501, E4030, The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of The ONE-Glo™ EX Luciferase Assay System quantitates firefly luciferase expression in multiwell plates using a simple add-mix-read protocol. As a result, these assays are difficult to establish in quality-controlled drug development settings. ONE-Glo™ Luciferase Assay 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM046 · Revised 9/23 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM058 · Revised 1/23 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM046 · Revised 9/23 . Normalized firefly luciferase activity (firefly luciferase www. Bright If you are using the 10ml size of Bio-Glo™ Luciferase Assay System (Cat. Includes: • 100ml Bright-Glo™ Luciferase Assay Buffer • 1 2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM033 · Revised 6/15 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM058 · Revised 1/23 The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System allows sensitive detection of firefly and NanoLuc luciferases. Renilla Luciferase Assay System has comparable sensitivity to the Luciferase Assay System (firefly luciferase) and 100-fold greater sensitivity than a published method for assaying PDF-1. eenkso ucdmu cfhk nzug csdu atea waje dch zrmxtyee gqpio